Israel will only have one singles player at a Grand Slam tournament after Amir Weintraub and Julia Glushko both lost their second qualifying match on Thursday evening.
Weintraub was looking to reach the main draw at Flushing Meadows for the first time, though was beaten in straight sets, Czech Radek Stepanek, 6-2, 7-6.
Glushko, who was looking to reach her fourth US Open, was also beaten in straight sets, beaten 6-4, 6-0 by American Jennifer Brady.
However, American Noah Rubin is just one win away from reaching the main draw after he beat German Michael Berrer 6-2, 4-6, 6-4. He next faces Russian Karen Khachanov.
The tournament gets underway on Monday.